Scalable software platform for advanced "Proteomics 2.0" analysis

The SORCERER^TM platform offers optimized SEQUEST®-based workflows for high-throughput data analysis of proteins and their post-translational modifications using mass spectrometry. It is designed for robust, high-sensitivity proteomics analyses commonly employed for biomarker discovery and for signal transduction research in cancer, stem cells, and neurodegenerative diseases.

SORCERER Enterprise

SORCERER Enterprise is the software version of the SORCERER platform. SORCERER Enterprise Base Edition software, running on one server (the head server) of a cluster or blade-server in an IT-maintained data center, provides the SORCERER workflows. It works with up to sixteen slave servers running the SORCERER Enterprise Plus Edition software, which execute the compute-intensive scoring modules. SORCERER Enterprise Base Edition can also be configured for a standalone server with no slave nodes, in which case the scoring module is run within the head server. Both software editions can be purchased pre-installed and tested on industry-standard servers for simplified deployment and technical support.

Benefits

• Scalable, parallelized SORCERER-SEQUEST searching
• Optimized algorithms for post-translational modification (PTM) analysis
• Multicore, 64-bit ready software, for best system performance and fit
• Robust Linux-based system
• Head server available preconfigured with software for faster deployment
• Slave servers for accelerated SEQUEST scoring
• Multi-user, web-based workflows with SORCERER technology
• Customizable MUSE^TM results post-processing and PTM characterization
• Supports all major MS-MS vendors’ file formats
• Searches Thermo RAW files directly
• Expandable data repository for spectral data and results

The intuitive Sorcerer Enterprise software offers different workflows for searching your data and viewing your results. The figure shows the Web-based GUI (upper left), which can be browsed from any desktop on the local area network, as well as the ProteinProphet report (upper right) of ISB’s Trans-Proteomic Pipeline and the summary view of Scaffold (below).


Product Specifications

SORCERER Enterprise Base Edition

• Sorcerer workflow software, including:
• Web-based Graphical User Interface with multi-user access
• PTM-enhanced SORCERER-SEQUEST algorithm
• MUSE postprocessing scripting environment, with Ascore phosphosite analysis
• Multi-vendor file format support, including: mzXML, .dta/.out and SRF (Bioworks 3.3.1) formats
• .pkl and .mgf for Waters, Bruker, Agilent and ABI connectivity
• Direct Thermo RAW file extraction (requires separate Xcalibur library installation)
• Pre-integrated Trans-Proteomic Pipeline (ISB)
• Available integrated Scaffold Batch protein ID (includes X!Tandem search engine)
• Optional Phenyx® protein identification package

Server requirements:

• Dual quad-core Intel Xeon processor
• Minimum 8GB RAM
• Minimum 2 Terabyte hard disk or external RAID drive
• DVD-RW optical drive; USB port
• Dual port 10/100/1000 Base-T Ethernet
• Redhat Enterprise Linux 5 operating system preferred, Suse Linux Enterprise Server 10 optional

SORCERER Enterprise Plus Edition
There may be up to sixteen identically configured slave servers, connected by a Gigabit Ethernet switch on a private LAN to the head server. The slave node subsystem may either be provided by the customer, or, for easier provisioning and support eligibility, acquired from Sage-N Research.

Minimum recommended server specifications:

• Intel Xeon dual-core CPU or equivalent, 2GHz or higher (64-bit ready)
• 1GB or more of RAM
• 100 GB or more available disk space
• 1000 Base-T Ethernet port and DVD-ROM
• Redhat Enterprise Linux 5 operating system preferred, Suse Linux Enterprise Server 10 available

Total Support Plan

Comprehensive support is available when the client purchases both hardware and software from Sage-N Research. An initial warranty is provided, and continuing coverage is available by subscribing to the Total Support Plan. Benefits include online and telephone software support, access to new releases (not including significant new functionality) of the software, and a hardware extended warranty.

Related Products

SORCERER^TM Integrated Data Appliance

The SORCERER 2 IDA is a fully integrated hardware/software system for proteomics data analysis. The application software, Sorcerer Proteomics Edition, is functionally similar to that of Sorcerer Enterprise, except that SEQUEST acceleration is internal to the appliance rather than using external compute nodes. Sorcerer is a plug-and-play solution for moderate to high throughput proteomics laboratories who need to be up and running with minimal IT intervention.

Scaffold Batch and Scaffold

Scaffold Batch (for server automation) and Scaffold (for desktop computer interactivity) are powerful and intuitive software tools for protein ID validation and analysis, multi-sample comparison and results reporting. Sage-N Research offers and recommends Scaffold Batch as an option for SORCERER Enterprise, and suggests installing Scaffold or the free Scaffold Viewer on client desktops that access the server.

Phenyx Server

Phenyx is an advanced protein characterization platform incorporating accurate, true probabilistic scoring. Phenyx Server software, available from Sage-N Research, may be co-installed on a SORCERER Enterprise server and interoperates with the SORCERER workflow software for increased results confidence in a multi-algorithm data flow.