Plug-and-play "Proteomics 2.0" Integrated Data Appliance

Sorcerer^TM integrated data appliances provide "plug-and-play productivity" in protein ID and characterization, with increased accuracy and throughput, and reduced IT maintenance. The flagship server version, Sorcerer 2, is designed to support multiple researchers, such as in high-throughput laboratories, core facilities and corporate environments. It is for scientists whose throughput and sophistication needs outgrow their basic all-in-one proteomics software, while offering improved reliability and operating cost compared to cluster computers.

High throughput, easy to use

Sorcerer 2 is a fully integrated data analysis system that is compact enough to fit under a desk, yet powerful enough to run the most rigorous algorithms needed for successful pathway proteomic studies. Sensitive and comprehensive SEQUEST® searches with multiple PTMs enabled become routine, even for large datasets. Results analysis is available with Scaffold, the Trans-Proteomics Pipeline or Bioworks®. Sorcerer increases your productivity with: thousands of spectra per hour throughput; the increased accuracy from multiple algorithms; and comprehensive support through our Total Support Program. With automated raw file searching and patent-pending indexing acceleration of SEQUEST searches, Sorcerer 2 makes proteomics data analysis quick and easy.

Benefits

• High throughput
• No IT maintenance
• Plug and play setup
• Simple operation
• Multi-user, web-based workflows
• Scriptable MUSE™ results post-processing and PTM characterization
• Supports all major MS-MS vendors’ file formats
• Search Thermo RAW files directly
• Optimized for PTM analysis
• Data repository for spectral data and results
• Robust Linux-based multicore system

Related Products

Sorcerer Enterprise
Scalable, customizable software platform with Sorcerer technology for large data centers.

Sorcerer XT
Sorcerer rack-mount format for server rooms, with upgraded system configuration and fibre channel interface for storage expansion.

Scaffold
Sorcerer rack-mount format for server rooms, with upgraded system configuration and fibre channel interface for storage expansion.

Phenyx Server
Phenyx, incorporating advanced protein characterization and true probabilistic scoring, is available from Sage-N Research and interoperates with SORCERER.

Specifications


Ready-to-use application software:

• Sorcerer workflow software, including:
• Web-based Graphical User Interface with multi-user access
• PTM-enhanced SORCERER-SEQUEST algorithm
• MUSE postprocessing scripting environment, with Ascore phosphosite analysis
• Multi-vendor file format support, including: mzXML, .dta/.out and SRF (Bioworks 3.3.1) formats
• .pkl and .mgf for Waters, Bruker, Agilent and ABI connectivity
• Direct Thermo RAW file extraction (requires separate Xcalibur library installation)
• Pre-integrated Trans-Proteomic Pipeline (ISB)
• Available integrated Scaffold Batch protein ID (includes X!Tandem search engine)
• Optional Phenyx® protein identification package

• Dual quad-core Intel Xeon processor (8 cores total)
• 8GB RAM
• High capacity disk storage (3.0TB or more) with RAID-5 redundant data archiving
• Network: Dual port 10/100/1000 Ethernet
• DVD-RW optical drive; USB port
• Dual port 10/100/1000 Base-T Ethernet
• Case dimensions (H×W×D): 18.6”×7.0”×30.3”
• Dimensions in stand: 19.0”×11.3”×30.5”
• Power: dual cable 100/240V autosensing
• Redhat Enterprise Linux 5 operating system

Phenyx

Phenyx is a software platform for the identification and characterization of proteins and peptides from mass spectrometry data. Developed by GeneBio in collaboration with the Swiss Institute of Bioinformatics (SIB) and incorporating the true probabilistic and flexible scoring system OLAV, Phenyx is specifically designed to meet the concurrent demands of high-throughput MS data analysis and dynamic results assessment.

Sage-N Research, Inc. (Sage-N) distributed GeneBio's Phenyx platform together with Sage-N's Sorcerer(TM) 2 IDA (Integrated Data Appliance) and as a standalone product as well.

Scaffold

Scaffold complements Sorcerer. High throughput confident protein identification is a two step process. First Sorcerer runs Sequest to match mass spectra to proteins. Then Scaffold validates these protein identifications. Scaffold first automatically validates Sequest results with the "prophet" statistical algorithms and by optionally cross checking Sequest with X! Tandem. Sage-N Research has bundled this part of Scaffold into their Sorcerer Integrated Data Appliance (IDA).

The automatically validated results can be distributed to collaborators who can do further validation manually with the free Scaffold Viewer. Scaffold Viewer provides a variety of ways of focusing the manual validation effort where it is most needed. The validation tactics available include visually checking for repeatability of the proteins across samples, peptides across samples, and the spectral fragmentation peaks across peptides. A more detailed tactic is to verify that the peptide is consistent with the fragmentation peaks in the spectra.

Scaffold complements Sorcerer by validating, organizing, visualizing, and publishing Sorcerer's data. Together Sorcerer and Scaffold provide a complete solution to MS/MS data analysis. Sage-N Research distributes Scaffold as an integrated part of Sorcerer 2.

Scalable software platform for advanced "Proteomics 2.0" analysis

The SORCERER^TM platform offers optimized SEQUEST®-based workflows for high-throughput data analysis of proteins and their post-translational modifications using mass spectrometry. It is designed for robust, high-sensitivity proteomics analyses commonly employed for biomarker discovery and for signal transduction research in cancer, stem cells, and neurodegenerative diseases.

SORCERER Enterprise

SORCERER Enterprise is the software version of the SORCERER platform. SORCERER Enterprise Base Edition software, running on one server (the head server) of a cluster or blade-server in an IT-maintained data center, provides the SORCERER workflows. It works with up to sixteen slave servers running the SORCERER Enterprise Plus Edition software, which execute the compute-intensive scoring modules. SORCERER Enterprise Base Edition can also be configured for a standalone server with no slave nodes, in which case the scoring module is run within the head server. Both software editions can be purchased pre-installed and tested on industry-standard servers for simplified deployment and technical support.

Benefits

• Scalable, parallelized SORCERER-SEQUEST searching
• Optimized algorithms for post-translational modification (PTM) analysis
• Multicore, 64-bit ready software, for best system performance and fit
• Robust Linux-based system
• Head server available preconfigured with software for faster deployment
• Slave servers for accelerated SEQUEST scoring
• Multi-user, web-based workflows with SORCERER technology
• Customizable MUSE^TM results post-processing and PTM characterization
• Supports all major MS-MS vendors’ file formats
• Searches Thermo RAW files directly
• Expandable data repository for spectral data and results

• Sorcerer workflow software, including:
• Web-based Graphical User Interface with multi-user access
• PTM-enhanced SORCERER-SEQUEST algorithm
• MUSE postprocessing scripting environment, with Ascore phosphosite analysis
• Multi-vendor file format support, including: mzXML, .dta/.out and SRF (Bioworks 3.3.1) formats
• .pkl and .mgf for Waters, Bruker, Agilent and ABI connectivity
• Direct Thermo RAW file extraction (requires separate Xcalibur library installation)
• Pre-integrated Trans-Proteomic Pipeline (ISB)
• Available integrated Scaffold Batch protein ID (includes X!Tandem search engine)
• Optional Phenyx® protein identification package

• Dual quad-core Intel Xeon processor
• Minimum 8GB RAM
• Minimum 2 Terabyte hard disk or external RAID drive
• DVD-RW optical drive; USB port
• Dual port 10/100/1000 Base-T Ethernet
• Redhat Enterprise Linux 5 operating system preferred, Suse Linux Enterprise Server 10 optional

SORCERER Enterprise Plus Edition
There may be up to sixteen identically configured slave servers, connected by a Gigabit Ethernet switch on a private LAN to the head server. The slave node subsystem may either be provided by the customer, or, for easier provisioning and support eligibility, acquired from Sage-N Research.

• Intel Xeon dual-core CPU or equivalent, 2GHz or higher (64-bit ready)
• 1GB or more of RAM
• 100 GB or more available disk space
• 1000 Base-T Ethernet port and DVD-ROM
• Redhat Enterprise Linux 5 operating system preferred, Suse Linux Enterprise Server 10 available

Total Support Plan

Comprehensive support is available when the client purchases both hardware and software from Sage-N Research. An initial warranty is provided, and continuing coverage is available by subscribing to the Total Support Plan. Benefits include online and telephone software support, access to new releases (not including significant new functionality) of the software, and a hardware extended warranty.

SORCERER^TM Integrated Data Appliance

The SORCERER 2 IDA is a fully integrated hardware/software system for proteomics data analysis. The application software, Sorcerer Proteomics Edition, is functionally similar to that of Sorcerer Enterprise, except that SEQUEST acceleration is internal to the appliance rather than using external compute nodes. Sorcerer is a plug-and-play solution for moderate to high throughput proteomics laboratories who need to be up and running with minimal IT intervention.

Scaffold Batch and Scaffold

Scaffold Batch (for server automation) and Scaffold (for desktop computer interactivity) are powerful and intuitive software tools for protein ID validation and analysis, multi-sample comparison and results reporting. Sage-N Research offers and recommends Scaffold Batch as an option for SORCERER Enterprise, and suggests installing Scaffold or the free Scaffold Viewer on client desktops that access the server.

Phenyx Server

Phenyx is an advanced protein characterization platform incorporating accurate, true probabilistic scoring. Phenyx Server software, available from Sage-N Research, may be co-installed on a SORCERER Enterprise server and interoperates with the SORCERER workflow software for increased results confidence in a multi-algorithm data flow.